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Second day @Ceramics_UK in Telford

The final day @Ceramics_UK & @MaterialsShow in Telford has been even better than yesterday. Many good and interesting discussions on how our Freeze Granulation technology can make an impact in developing a sustainable future for the ceramics industry and for the globe. #CUK19 #AMS19

Second day at Ceramics UK 2019.
Second day at PowderPro’s stand at Ceramics UK 2019.

First day at Ceramics UK in Telford

During the first day at the @Ceramics_UK we have had good discussions with researchers at companies and universities on how to apply Freeze Granulation on their different materials. #CUK19.

PowderPro at Ceramics UK

Atmospheric spray freeze drying of sugar solution with phage D29

Atmospheric spray freeze drying of sugar solution with phage D29

Alvin Ly, Nicholas B. Carrigy, Hui Wang, Melissa Harrison, Dominic Sauvageau, Andrew R Martin, Reinhard Vehring, Warren H Finlay

Abstract

Therapeutic bacteriophages offer a potential alternative approach in the treatment of drug resistant bacteria. In the present study, we examine the ability of atmospheric spray freeze-drying (ASFD) to process bacteriophage D29 into a solid dry formulation. Bacteriophage D29 is of particular interest due to its ability to infect Mycobacterium tuberculosis. A sugar solution containing bacteriophage D29 was sprayed and instantly frozen in a cold chamber. Cold drying gas was then passed through the chamber at a high flow rate and atmospheric pressure. Convective transport combined with the low temperature of the drying gas results in sublimation of ice, yielding a free-flowing, porous powder. The bacteriophages were atmospheric spray freeze-dried in solutions with varying concentrations of trehalose and mannitol. A solution of trehalose and mannitol at a mass ratio of 7:3 and a total mass concentration of 100 mg/mL led to powder with 4.9 ± 0.1% moisture content and an acceptable titer reduction of ∼0.6 logs. In comparison, a pure trehalose solution and a 1:1 ratio of trehalose and mannitol both had titer reductions of >1.5 logs. Spectroscopic analysis showed that trehalose in the powder was amorphous while mannitol completely crystallized during the drying process, both of which are desirable for preserving phage viability and storage in powders. The results highlight the potential for using ASFD as an alternative process in preserving biopharmaceutical products.

Inert particles as process aid in spray-freeze drying

Inert particles as process aid in spray-freeze drying

Fan Zhang, Xiaoyu Ma, Xiusheng Wu, Qing Xu, Wei Tian, Zhanyong Li

Abstract

Spray-freeze drying (SFD) is a novel and advanced drying technology in the production of high-value foods and pharmaceuticals. However, long drying time is still a disadvantage for the SFD applications. This constraint could be alleviated using inert particles. This article provides the experimental results on SFD of whole milk in an integrated spray-freeze and vacuum freeze drying equipment. The effects were quantified in terms of the SFD drying time and the properties of the obtained milk powder in relation to the size of stainless steel balls used as inert particles. It is shown that the drying time could be reduced to some extent due to inert particles embedded and the milk powder of reduced size has high porous microstructure.

Third day at ECerS 2019 in Torino

Good discussions on the application of Freeze Granulation on different ceramic powder systems.

Discussions on Freeze Granulation at PowderPro stand at #ECerS 2019 in Turin.

Second day at ECerS 2019 in Turin

Interesting discussions on applications of Freeze Granulation on ceramic powders at ECerS 2019 in Turin.

PowderPro stand at ECerS 2019 in Turin.

First day at ECerS 2019 in Turin

Discussions on how to apply Freeze Granulation on diffrent ceramic powder systems.

PowderPro at ECerS 2019 in Turin.

Dry powder inhalation of siRNA

Dry powder inhalation of siRNA

Tobias WM Keil, Olivia Merkel

Keywords

Dry powder inhaler, Inhalation, Nanoparticle, siRNA, Spray drying, Spray freeze drying

Development of Inhalable Dry Gene Powders for Pulmonary Drug Delivery by Spray-Freeze-Drying

Development of Inhalable Dry Gene Powders for Pulmonary Drug Delivery by Spray-Freeze-Drying

Edina Vranic, Merima Sirbubalo, Amina Tucak, Jasmina Hadžiabdić, Ognjenka Rahić, Alisa Elezovic

Abstract

There is considerable potential for pulmonary gene therapy as a treatment for a number of conditions for which current treatment is inadequate. Delivering genes directly to the lungs by dry powder inhalers (DPIs) have attracted much attention due to better stability of genes. Formulating genes as powders for aerosol delivery is a challenge as it requires not only flowability and dispersibility of the powders but also maintaining gene stability and biological activity during manufacturing and delivery. In this review, we aim to provide an overview about the potentials of spray-freeze-drying (SFD) for the development of inhalable dry gene powders for pulmonary drug delivery. We will discuss the main steps involved within the production process (i.e., spraying, freezing and drying) and introduce different SFD methods which can successfully be used for the production of porous particles whose physical and aerosol characteristics are considered to be ideal for use in pulmonary drug delivery.

Establishment of evaluation method for gene-silencing by serial pulmonary administration of siRNA and pDNA powders: Naked siRNA inhalation powder suppresses luciferase gene expression in lung

Establishment of evaluation method for gene-silencing by serial pulmonary administration of siRNA and pDNA powders: Naked siRNA inhalation powder suppresses luciferase gene expression in lung

Takaaki Ito, Tomoyuki Okuda, Ryo Takayama, Hirokazu Okamoto

Abstract

In order to evaluate the in vivo effect of inhaled formulations, it is a gold standard to create a lung metastasis model by intravenously injecting cancer cells into an animal. Because the cancer grows from the blood vessel side, there is a possibility of underestimating the effect of an inhaled formulation administered to the lung epithelium side. In addition, the metastasis model has disadvantages in terms of preparation time and expense. The present study aimed to establish a new method to evaluate the effect of an inhaled small interfering RNA (siRNA) formulation that is more correct, more rapid, and less expensive. We investigated whether siRNA can suppress gene expression of plasmid DNA (pDNA) by serial pulmonary administration of siRNA and pDNA powders prepared by spray-freeze-drying. We revealed that formulations of dry siRNA powder significantly suppressed gene expression of pDNA powder compared with a control group with no siRNA. Naked siRNA inhalation powder with no vector showed the suppression of gene expression equivalent to that of an siRNA-polyethyleneimine complex without damaging tissues. These results show that the present method is suitable for evaluating the gene-silencing effect of inhaled siRNA powders.