Membrane‐Freeze Concentration Hybrid for Temperature‐Sensitive Biomolecules. Investigation, Application, and Techno‐Economic Benefits

Membrane‐Freeze Concentration Hybrid for Temperature‐Sensitive Biomolecules. Investigation, Application, and Techno‐Economic Benefits

Timo van Beek, Michael Budde, Jan van Esch

Abstract

In order to close the technology gap between membrane technologies and spray/freeze‐drying ideally with a technology that avoids thermal stress to sensitive enzyme solutions, the limits of freeze concentration for this application have been investigated. On laboratory scale it was found that average crystal sizes are > 300 µm despite high viscosity and ice separation is possible up to 42 % solids and > 1000 mm2s−1 viscosity. No activity loss was observed during concentration. A combination of two‐stage freeze concentration with a filter and wash column for ice liquid separation in an integrated setup with ultrafiltration has the greatest potential and was shown to be economically feasible in three out of four cases studied.

Keywords

Biomolecules, Crystal size, Freeze concentration, Freeze‐drying, Membrane separation, Sensitive enzymes

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